Fors6601 Practical Assignment 5

Instructions:

1. Using the information from the following matrix, make a family tree using LucidChart or draw.io and take a screenshot.

Question 1: According to the The Shared cM Project 4.0 tool beta v4 (https://dnapainter.com/tools/sharedcmv4-beta), which of the following are the most likely relationships between A and E? (Multiple choices)

A. siblings

B. 1C

C. aunt/uncle – niece/nephew

D. grandparent/grandchild

E. 1C1R

Question 2: According to the The Shared cM Project 4.0 tool beta v4 (https://dnapainter.com/tools/sharedcmv4-beta), which of the following are the most likely relationships between B and E? (Multiple choices)

A. 1C

B. 1C1R

C. siblings

D. aunt/uncle – niece/nephew

E. great-grandparent/great-grandchild

Question 3: Looking at the family tree you created from the matrix, what is the relationship between A and C?

A. grandparent – grandchild

B. sibling

C. half-sibling

D. aunt/uncle – niece/nephew

Question 4: Looking at the family tree you created from the matrix, which of the following is D related to? (Multiple choices)

A. C

B. B

C. E

D. A

Question 5: Looking at the family tree you created from the matrix, what is the relationship between B and C?

A. grandparent – grandchild

B. aunt/uncle – niece/nephew

C. great-grandparent/great-grandchild

D. half-sibling

2. Below are the trees of 4 DNA matches to Therese Laporte, born in 1957. We would like to identify her parents.

i. Find the most recent common ancestor of these matches

ii. Make a WATO+ which includes these matches and the amount of DNA they share with Therese.

iii. Make sure that in the settings, none of the 4 boxes at the top are checked.

iv. Click on suggest hypotheses. You should have 10 hypotheses.

v. Click on “settings and actions” to save an image of this WATO+

HINT: Do not forget to define half-relationships!

Question 6: Looking at the family trees that were given for the WATO+, what is the relationship between Samuel and Josette?

A. 1C

B. 2C

C. 1C1R

D. siblings

Question 7: Looking at the family trees that were given for the WATO+, what is the relationship between Charlotte and Samuel?

A. 1C1R

B. 2C

C. siblings

D. 1C

Question 8: True or False: Looking at the WATO+, it is possible that Therese is a child of Joseph.

Question 9: What is the percent likelihood of the highest hypothesis on the WATO+?

Question 10: Looking at the WATO+, what is the percent of the hypothesis that is 16 times more likely than the next hypothesis?

3. Make a DNA Painter chromosome map for kit JL3742941 (Gedmatch: https://app.gedmatch.com/select.php).

i. Add match DS1306954 to the maternal side. You can use the match’s name as the name of the ancestor.

ii. Screenshot all the shared segments with kit DS1306954 .

iii. Run the Gedmatch Tier 1 tool Segment Search tool for kit JL3742941

iv. One by one, add matches A206100, WP7591667, NH8638716, M986019 and KJ5165618 to the map. Select “I don’t yet know how I’m connected to this match.” Be sure to add the name of the match. Use the name of the match for the name of the group

v. Take a screenshot of “Shared or Both” for Chromosome 1.

Question 11: Looking at the DNA Painter chromosome map, on which chromosomes does kit DS1306954 share DNA segments? (Multiple choices)

A. 12

B. 8

C. 18

D. 1

E. 21

F. 4

Question 12: Looking at the DNA Painter chromosome map, how many segments does kit DS1306954 share on chromosome 8?

Question 13: True or False: Looking at the DNA Painter chromosome map for Chromosome 1, Ann, Cylmara, and Caroline share a common ancestor.

Question 14: Looking at the DNA Painter chromosome map for Chromosome 21, how many segments are mapped?

Question 15: Looking at the DNA Painter chromosome map for Chromosome 1, the name Nikki DeMartini appears twice. Why?

A. She has a triangulated segment with herself

B. There was an error in the program

C. She uploaded her kit twice to Gedmatch

D. She is a very important match